While the meiotic transcriptional program and pre-meiotic DNA synthesis were largely unchanged, the meiotic divisions were strongly inhibited. Finally, we found that the neo1D/NEO1 heterozygous strain is highly sensitized to TA in sporulating cultures. Neo1 is involved in intracellular membranetrafficking, protein sorting and vacuole biogenesis. Temperaturesensitive mutants of neo1 have been shown to exhibit fragmented… Continue reading Gene important for both sporulation and autophagy exhibits phenotypes similar to those observed in TA-treated yeast undergoing sporulation
LEKTI-2 and KLK5 expression are accordable to those results but need further evaluation by electron microscopy
The surface-exposed epidermis, a self-renewing stratified squamous epithelium composed of several layers of keratinocytes, is most important for the barrier defense against these challenges. Keratinocytes in the outmost stratum corneumof the epidermis are shed off and replaced by newly differentiated cells originating from epidermal stem cells located in the basal layer. They undergo a specific… Continue reading LEKTI-2 and KLK5 expression are accordable to those results but need further evaluation by electron microscopy
Peptides arising from cleavage were detected in the cellular peptidome when cells were treated for epoxomicin
Plk1-PBD and checked for good hydrogen bond interactions with the five key residues. This resulted in the identification of 526 compounds with good hydrogen bonding. Figure. 6 represents the binding orientation of one hit compound within the Plk1-PBD and also how well the compound fits into Hypo1. To further narrow down the candidate list we… Continue reading Peptides arising from cleavage were detected in the cellular peptidome when cells were treated for epoxomicin
To facilitate comparison of the new data with previous results using different proteasome inhibitors
To reduce the contribution from secondary changes due to altered protein levels, cell stress, or cell death; these do not occur upon short exposure of cells to proteasome inhibitors. Peptide levels were measured using a quantitative peptidomics technique that uses stable isotopic labels to compare up to five samples in a single experiment. For all… Continue reading To facilitate comparison of the new data with previous results using different proteasome inhibitors
Characterized by low functional and antigen plasma levels of C1-inh that can arise
Whereas HAE type II patients are characterized by low functional, but normal or increased antigen C1-inh plasma levels. This classification has however been challenged by observations of intermediary HAE types, when small amounts of dysfunctional C1-inh is present in the blood stream. As no evidence regarding clinical consistencies between the type I and type II… Continue reading Characterized by low functional and antigen plasma levels of C1-inh that can arise