In summary, we have demonstrated that significantly increased expansion of cord blood-derived stem cells is possible using functional nanofibers and serum-free culture media. These expanded stem cells retain their progenitor cell phenotype and provide an opportunity to genetically manipulate them for cellbased therapy. We have also shown that non-viral delivery of proangiogenic factors VEGF and PDGF markedly enhanced the angiogenic effects of stem cells by increasing tissue expression of connexin 43 and growth factors related to angiogenesis. This study thus confirms the feasibility of combined stem cell therapy with pro-angiogenic gene therapy. Furthermore, it also identifies the therapeutic potential of nanofiber-expanded stem cells in treating ischemic heart disease. Furthermore, certain SA clinical strains have recently evolved resistance to vancomycin, an antibiotic to which staphylococci have previously been uniformly sensitive. Although the vancomycin-resistant strains remain rare, Z-Ligustilide methicillin-resistant strains are increasingly common, highlighting the urgent need to develop new approaches for the treatment of SA infections. Furthermore, the incidence of community acquired strains of MRSA has markedly increased over the past several years. Topically applied nitric oxide is a potentially useful preventive and therapeutic strategy against superficial skin vitexicarpin infections, including MRSA infections. In the healthy state and under pathologic conditions, it is well established that NO maintains skin homeostasis by regulating circulation, ultravioletmediated melanogenesis, sunburn erythema, and the maintenance of the protective barrier against microorganisms. Notably, NO modulates immune responses and is a significant regulator of wound healing. We have recently developed an inexpensive and stable NO releasing platform using nanotechnology based on a silane hydrogel. Moreover, our platform benefits from the presence of chitosan, which also has antimicrobial activity. Chitosan is a polymer derived from crustacean exoskeletons that binds to and disrupts the cell wall and membrane of microorganisms due to its cationic charge in weakly acidic environments.