For example, during interphase mammalian Mps1 kinases locate to centrosomes and regulate their duplication but during mitosis they locate to kinetochores where they are required to activate the spindle assembly checkpoint. The SAC helps ensure faithful mitosis by delaying anaphase onset until all kinetochores are correctly attached to the spindle and recruitment of SAC proteins to mitotic kinetochores is a hallmark of SAC activation. Moreover, the human Prp4 kinase was unexpectedly found to locate to mitotic kinetochores and subsequently shown to function in the SAC although it was previously thought to function only in RNA processing. Thus changes in the location of kinases from interphase to mitosis are often predictive of their function. A. nidulans offers many advantages for proteomic studies defining protein interactions and subcellular localizations. Aiding biochemical analysis is the ability to produce an enormous number of genetically identical conidia for the rapid generation of biomass which can easily be harvested by filtration. Methods are in place for single step affinity purification of proteins endogenously tagged with the 15 amino acid S-tag using S-protein beads for downstream mass spectrometry analysis to identify associated proteins, define post-translational modifications and complete biochemical assays. A. nidulans is also compatible with live cell imaging of proteins endogenously tagged with fluorescent markers and attaches to glass coverslips helping facilitate imaging over long time periods of polarized growth. To streamline proteomic analysis in A. nidulans we describe here dual localization-affinity purification tags comprised of GFP in tandem with the S-tag for endogenous C-terminal or Nterminal protein tagging. To establish the utility of DLAP tags and advance the understanding of Fingolimod Src-bcr-Abl inhibitor fungal kinase biology, we have endogenously tagged, localized and affinity purified 17 A. nidulans protein kinases and completed mass spectrometry analysis for 10 affinity purified kinases. Analysis of this data demonstrated that DLAP tagged versions of the CotA, SldABub1/R1 and NimXCdk1 kinases each specifically co-purify associated proteins. Comparative analysis of interphase and mitotic localizations indicated that 13 kinases changed location from interphase to mitosis and 7 kinases located to mitotic structures. Our data suggest functions for the An-Cdc7 DNA replication kinase at mitotic SPBs, the TorA kinase at endomembranes and provide novel insights into how SepH kinase location to SPBs differentially regulates septation generating asymmetric cell divisions along the length of hyphae. The anti-inflammatory actions of NSAIDs are carried out through the inhibition of cyclooxygenase-1, which diminishes the biosynthesis of prostaglandins and deviates the metabolism of arachidonic acid towards the formation of pro-inflammatory cysteinyl-leukotrienes, thus triggering a hypersensitivity response in susceptible individuals.