Recently, honey has been tested and approved scientifically for its functional and biological properties such as anti-oxidant, anti-inflammatory, anti-bacterial, anti-viral, anti-ulcerous activities and anti-cancer properties. Honey may provide a basis for the development of novel adjuvant for patients with glioma. Recent studies showed a significant anticancer activity of Malaysian jungle honey on human breast, cervical, oral and osteosarcoma cancer cell lines. Research also showed an anti-proliferative activity of other honeys, like honeys from Manitoba in bladder cancer, honeys from Iran in renal cell ABT-199 carcinoma, manuka honey in human breast cancer cell, murine melanoma cell. However, until now honey has not been found in studies to show antiproliferative effects on glioblastoma cancers. Brain tumors are the second leading cause of cancer related deaths in males up to the age of 39 and in females and children younger than 20 years. Glioblastoma multiforme is the most aggressive form of brain tumor. Despite standard treatments consisting of surgery, postoperative radiotherapy and temozolomide, patient survival remains poor, mainly attributed to tumor inherent radio- and chemoresistance. Despite the continuous improvements in the treatment of GBM during the past decade, these tumors are still associated with a poor prognosis and a rare long-term survival of the patients. Therefore, there is a great need to understand the underlying mechanisms of tumor progression in order to define novel therapeutic targets for GBM. To the best of our knowledge, this work presents, for the first time, the anticancer effect of honeys from Poland on tumor GBM cell line. The current study is aimed at investigating the anti-proliferative and anti-metastatic effect of honeys on GBM cancer cells. Interaction between honey and TMZ was also estimated. Furthermore, we performed a qualitative analysis of the tested honeys. GBM is the most malignant form of primary astrocytic brain tumors in adults. Patients with GBM usually undergo a treatment which consists of the combinations of surgery, radiotherapy and chemotherapy but have a median survival time of less than one year. TMZ is the most commonly used for patients with GBM and this medicament has proven to improve prognosis. Recently, many patients suffering from cancer or other chronic conditions have been looking for natural products and the use of alternative medicine. Honey has been recognized by many ancient cultures for its healing properties, and recently has been investigated as an anticancer agent. The anti-cancer activity of honeys has been analyzed for bladder cancer Manitoba, renal cell carcinoma, human breast cancer cell and murine melanoma cell. To the best of the authors’ knowledge, our research is the first to show the anti-proliferative effects of honey on glioblastoma cell line. In this study, a cytotoxic activity of the examined four samples honey showed a strong cytotoxicity of H4 and H1, and this may be connected with the content of polyphenols in honeys, because the samples H1 and H4 have a much higher TPC than H2 and H3.