Although the mutants showed no difference from the wild-type protein, these negative results only indicate that the variations did not affect these specific features in certain cells. Indeed, the variants might impair the function of DLC1 in other ways or in other cardiac cells. Furthermore, to the best of our knowledge, this is the first report using in vitro assays to demonstrate that DLC1 isoform 1 manifests a function analogous to isoform 2. In conclusion, our mutational analysis of DLC1 isoform 1 presents a spectrum of rare variants in a CHD cohort and shows a mutation cluster in the N-terminus of the DLC1 protein. Our functional assays prove that the ability to inhibit cell migration or the subcellular localization of the protein are altered by three private variants. These findings provide novel insight that DLC1 may be a high-priority candidate gene associated with CHD.. ASD, which occur in 1 out of 150 individuals, include different Dinaciclib neurodevelopment disorders that manifest mainly in the earlier years of life, affecting language, communication and reciprocal social interaction development. Risp has low solubility in aqueous medium and, when orally administered, exhibits low bioavailability due to extensive first-pass metabolism and high protein binding. Moreover, non-targeted delivery usually results in numerous side effects. Since Risp target organ is the brain, it is necessary not only to develop a strategy to improve drug bioavailability, by avoiding first-pass metabolism, but also to achieve the desired drug concentration at the site of action, thus reducing undesirable side effects. In the last years, strategies with chemical therapies, particularly the design of nanostructured drug carrier systems, have been proposed to overcome these issues regarding ASD treatment. However, these kinds of carriers must be carefully designed and/or chosen because their pharmacokinetics, biodistribution, and tissue selectivity depend exclusively on the nanocarrier structure. In this sense, dendrimers are exceptional polymers presenting important advantages over conventional linear or branched ones such as polyethylene terephthalate or comb polymers, respectively. These advantages include monodispersity, controlled size in the range of nanometers, controlled number of surface groups, and extremely high area/volume ratio. Only intermediate generation dendrimers are suitable drug carriers, with structures open enough to enable the loading and subsequent release of molecules in a controlled fashion. Since, in the last years, PAMAM dendrimers have been found to be useful to improve the solubility of low aqueous soluble drugs, the present work aims to enhance Risp solubility by means of PAMAM dendrimers. On the other hand, we used the zebrafish as an ideal model to study developmental neurobiology and other fields of biomedicine. The zebrafish is a teleost of the Cyprinid family, with several advantageous features for use in the laboratory: its small size allows easy maintenance of several individuals with relatively low costs; females lay a large number of eggs; embryos develop rapidly and are semitransparent 24 hours post-fertilization.