With the aim of developing a method for controlling the quality and quantity of crawfish and their eggs in aquaculture, many studies have been investigated on crawfish gonadal development. Knowledge of mechanisms governing gonadal development processes at the molecular level is crucial and could be directly applied to the crawfish industry. Transcriptome sequencing provides general representation of almost all transcripts expressed in specific cells or organs at particular conditions and stages. Because of its advantages of high throughput rates and low costs, the 454 pyrosequencing technology is regarded as the first choice for the identification of novel genes in organisms lacking a reference genome. It has thus been employed for other crustacean species, including Litopenaeus vannamei, Macrobrachium rosenbergii, M. nipponense and Euphausia superba. Screening and identifying gonadal differentially expressed genes are an initial step towards understanding gonadal development in P. clarkii. In the present study, we performed a 454 pyrosequencing of P. clarkii using prepared cDNA from mRNA isolated from testis and ovary tissues. This was used to generate expression profiles and to discover differentially expressed genes in these two tissue types. Based on previous research, and Dexmedetomidine HCl employing bioinformatics tools, we aimed to generate a list of candidate genes that may be involved in the gonadal development of P. clarkii. This could provide a major resource for future studies of crawfish gonadal development, and in particular should help to establish new ideas for the artificial regulation of reproductive processes in crawfish aquaculture. Respiratory viruses are associated with the majority of asthma exacerbations, which are a major cause of disease morbidity. Asthmatics do not appear to have more frequent viral infections than healthy individuals, but instead suffer more persistent and severe lower respiratory tract symptoms ; human rhinovirus infections are the most commonly identified in older children and adults.Given the importance of HRV in asthma and the Ascomycin paucity of effective anti-viral therapeutic options, a better understanding of the immune and inflammatory response to HRV is a significant focus of current respiratory research.