Magnetosomes, the organelles of magnetotactic bacteria, have nanometer-sized magnetic crystals surrounded by a lipid bilayer membrane and organize into chains via a dedicated cytoskeleton within the cell. Magnetosome proteins include approximately 30 proteins in M. gryphiswaldense MSR-1 and 78 proteins in M. magneticum AMB-1. These proteins are involved in the formation of magnetites. Mms 16, MpsA and Mms24 are responsible for mediating the invagination of the cytoplasmic membrane to form magnetosomes. MamJ and MamK are involved in magnetosome chain formation. MagA, MamB and MamM participate in iron transport into magnetosomes. Mms6 initiates magnetite crystal formation and/or morphological regulation. In honeybees, iron deposition begins on the second day after eclosion in the iron deposition vesicles of trophocytes. A cloudy layer just beneath the inner IDV membrane plays an important role in the formation of 7.5-nm spherical iron particles. Subsequently, iron granules are formed by orderly aggregation of the 7.5-nm spherical iron particles in the center of the IDVs. Finally, superparamagnetic magnetite is formed in the center of mature IGs. However, even though magnetite has been demonstrated in honeybees, neither the proteins involved in the formation of Sweroside the 7.5-nm spherical iron particles nor the proteins that convey these tiny particles to the centers of IDVs have been identified, nor has the iron deposition microenvironment of IDVs been characterized. In this study, we purified the proteins from IGs and IDVs and prepared their antibodies. We then use immunofluoresence-labeling, immunogold labeling, and co-immunoprecipitation techniques to examine the mechanism of magnetite biomineralization in the IDVs of honeybees. A previous study on magnetotactic bacteria showed that ferrous ion is present in the cytoplasm and the magnetosome in these bacteria and that this is responsible for carrying out magnetite biomineralization. Additionally, magnetite was formed in an artificial vesicle containing ferrous ion when the pH was increased from 4 to 12. Therefore, we speculate that ferrous ion may also be present in the cytoplasm in honeybees for the purpose of carrying out magnetite biomineralization. Ferrous ion would then be transported into the acidic space between the outer and inner IDV membranes. It has been observed that the Pulchinenoside A spherical iron particles spontaneously move to the center of IDVs in an orderly fashion. This observation suggests that a regular route for ferritin transport may exist in the lumen of IDVs and a putative actin-myosin-ferritin system may play the role of the transporter in this process. An actin chain serves as the route of transport with one end of a myosin molecule being attached to the actin chain and another end to ferritin, which allows myosin to carry ferritin along the actin chain to the center of IDVs. This reaction requires Ca2+ and ATP, and we also identified ATP synthase in purified IGs and IDVs. This putative system could reasonably explain the function of calcium and phosphate in IGs because energy dispersive X-ray spectrum analysis showed that iron, calcium, and phosphate were present in IGs. In magnetotactic bacteria, it has been demonstrated that an ATPase that is essential for iron trafficking is present in the cytoplasm. Thus the mechanisms by which mutations in this gene can induce dopaminergic cell death are a major focus of interest for those seeking to define the molecular pathogenesis of PD. The function of the PINK1 protein is not yet defined, although it is known to be targeted to mitochondria, a significant component of PD pathogenesis and is thought to be involved in protection against free radical generation.