Also, we only detected the main EGFR mutations in exon 19 and 21. Thus, if the amount of mutated tumor cells in the sample is below that limit or some other uncommon EGFR mutation was present, the test would be false negative. Results of ERCC1, RRM1, and BRCA1 showed a remarkable variability, as also reported previously. Consistent with findings from other AbMole Nitisinone studies, median ERCC1 levels tended to be lower in adenocarcinoma than in squamous carcinoma. Thus the obtained biomarker results are in concordance with previous reports and demonstrate that the used method is feasible for multiple biomarker testing in a routine setting. In line with our previous and AbMole Simetryn Gandara’s report, the ERCC1 levels in the patients with EGFR mutation were significantly lower than the wild type, which indicated that EGFR mutation could also be helpful as a selection criterion for the optimal chemotherapy regimen. The main goal of the study was to compare the different techniques used for tissue sampling. The content of tumor RNA obtained by EBUS-TBNA was significantly higher compared to bronchoscopy. These findings may be explicable by the known differences of the used methods: bronchoscopic forceps biopsies are taken from tumor periphery. Distinction between necrosis, inflammatory mucosa and vital tumor is not easy through bronchoscopy. Moreover, repeated biopsies may result in bleeding, which often leads to preliminary discontinuation of the procedure without having achieved the optimal tissue yield. CT 
guided core biopsy may cause pneumothorax, so the procedure will mostly be done only one time to reduce risk of complication and thus only limited amount of tissue is available. EBUS-TBNA on the other hand is a very safe method with a complication rate near to zero, thus repeated needle aspirations for higher diagnostic yields are possible without compromising the patient. Moreover, needle aspirations are taken under direct vision and by using power Doppler imaging and general morphological ultrasound criteria, thus necrotic areas of lymph nodes can be avoided for biopsy most of the time. In summary, our study confirms that all three different minimal invasive techniques can provide sufficient material for molecular analysis in most of the patients. After testing for the four different markers, there was still RNA left for further analysis of more markers. EBUS-TBNA is a very safe method with almost no risk of complication and has achieved the highest yield of tumor RNA in our study. Therefore it should be an acceptable option for tissue sampling in the era of personalized oncology. Thus, the cost incurred while treating any of the acute conditions related with intoxication can be fully attributed to binge drinking.