Author: BioactiveCompoundLibrary

Phosphorylation can rapidly and reversibly modulate numerous properties of proteins including their conformation, enzymatic activity, molecular interactions, subcellular localization and surface charge. In organisms as diverse as mammals, invertebrates and yeast, researchers have used mass spectrometry based proteomics to identify thousands of sites of phosphorylation on proteins in cell signaling pathways. Some sites of phosphorylation… Continue reading Interactions and molecular states involved in pheromone signaling phosphorylation of protein components of signaling pathways

CD44 receptor through a mechanism like receptor clustering. In previous studies, another CD44 binding peptide, A6, was found to enhance cell adhesion to HA and induce FAK and MEK phosphorylation in a CD44 dependent manner in breast and ovarian cancer cells. A6 also inhibited cancer cell migration and metastasis in vivo. It is unclear whether… Continue reading Certain epithelial cells loose cell-cell adhesion and invade the local tissue

T280 is required for Dig1mediated Ste12 activity: cells expressing Dig1-YFPT280A displayed a severely diminished transcriptional response, phenocopying the full deletion of Dig1. Strikingly, the closely situated residues T277 and S279 had the opposite effect in that they inhibit signal output: mutation of either residue leads to an increased pheromone response. Since these sites lack consensus… Continue reading Defining the specific functions of genes involved in cell wall biogenesis is important in order

We have not ruled out that the Mepiroxol altered pathway output we observed is due to structural changes unrelated to phosphorylation and/or altered protein/protein interactions caused by the alanine substitutions. In strains expressing a protein with phosphorylation site point mutations, no cell will have the mutated site phosphorylated. In the reference strain in any given… Continue reading The changes in pathway output are the direct consequence of the inability of the mutated residue to be phosphorylated

ABCA1 in the CNS increases apoE lipidation and greatly decreases amyloid deposits in AD mice. Transcription of ABCA1 and apoE is induced by agonists of Liver X receptors, which regulate many genes involved in lipid metabolism and inflammation. Genetic deficiency of LXRs increases amyloid burden in AD mice. Synthetic LXR agonists including TO901317 and GW3965… Continue reading To determine the therapeutic utility of LXR agonists for this type of brain injury